Removal of template DNA from cRNA preparations by combined oligo(dT) affinity chromatography and DNase I digestion
نویسندگان
چکیده
منابع مشابه
Digestion of insect chromatin with micrococcal nuclease, DNase I and DNase I combined with single-strand specific nuclease S1.
The chromatin of the lepidopteran Ephestia kuehniella was digested by micrococcal nuclease, DNase I and S1-nuclease combined with DNase I pretreatment. The resulting DNA fragments were analyzed by gel electrophoresis and compared with the DNA fragments of rat liver nuclei obtained by the same process. Extensive homology was revealed between insect and mammalian chromatin structure. The combined...
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porally correlated with the processes of chromosome segregation and nuclear envelope reformation. Our live image analysis with the multicolor cell line clearly indicated that survivin diffused into cytosol coincident with the chromosome segregation (435 min) and reappeared in the midzone during the nuclear envelope reformation (441–444 min). The separate images of ECFP-histone and EGFP-survivin...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2004
ISSN: 0736-6205,1940-9818
DOI: 10.2144/04363rn02